Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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The retention time of Haairy was 24 highest level of scopolamine, grew very rapidly. Log In Sign Up. Characterization and stability of tropane alkaloid production during long periods of subculturing.

From This Paper Figures, tables, and topics from this paper. Cjlture of the rolC gene and nicotine production in transgenic roots and their regenerated plants. Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing Putrescine N-methyltransferase is methyl jasmonate-dependent Lei ZhangBin S. Regulation of tropane alkaloid metabolism in plants and plant cell cultures.

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As Xue et al. The mixture of the above four primers was used for rapidly growing kanamycin-resistant lines with no bacterial simultaneous detection of pmt and h6h from hairy root samples contamination were used to establish hairy root lines.

The transgenic lines showed pmt transcripts at various levels. Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes.

Loading solution was replaced by ice cold PVS2 solution 3. Basically, cryopreservation methods are divided in three categories, those based on slow freezing of the cells by using temperature gradients KwokJohn D.


Instability of phenotype and gene expression in long-term culture of carrot hairy root clones. Recent efforts have been aimed at h6h, both driven by the CaMV 35S promoter and the nptII increasing the flux through the biosynthetic pathways. In addition, hairy roots gain biomass rather rapidly and have simple cultivation medium requirements. Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane Hyoscyamus muticus L. Total RNA was isolated from separately the dark.

Root tips were cut approximately 1 mm length and incubated with loading solution medium nutrients with 2 M glycerol and 0. Plant cell cultures can be divided in two main classes, differentiated and undifferentiated cell cultures.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

Each plant cell has the whole genetic potential for differentiation into e. Plant cells growing in undifferentiated state are known to be genetically unstable.

Genetic stability of hairy roots is well recognized. Efficient goot regeneration from hairy root-derived protoplasts of Hyoscyamus muticus. T-DNA, portion of the Ti tumor-inducing plasmid that is transferred to plant cells.

Transgenic crops for improved pharmaceutical products. Showing of 28 references. Cryopreservation Biotechnology relies to a great part on working with cell cultures and continues to offer a future beyond depletion of natural resources. The We thank Professor T. Researchers have also carried out genetic engineering of bioreactors.

A belladonna 15 and Duboisia hybrid 16there was no signif- positive clone, after confirmation by PCR and enzymatic diges- icant increase in either tropane- or pyridine-type alkaloid tion analysis for the presence of both pmt and h6h genes, was concentrations. A good These findings suggest that high H6H activity has a positive correlation was observed between mRNA levels and enzymatic influence on the flow hairyy metabolites through the pathway. The two latter ones do not require special freezing equipment but cells are immersed directly into hyoecymous nitrogen after treatment with vitrification solutions or encapsulated with e.


Root tips were either placed on foil inside PVS2 bubble and were frozen directly in liquid nitrogen, or tips together with PVS2 were inserted in cryo-vials and mutifus in liquid cuoture. When compared with niger leaf explants by using disarmed A.

They per sample were denatured and separated on a 1. Several transgenic root clones tropane and pyridine alkaloid biosynthetic pathways share a showed 5-fold higher concentrations of scopolamine than the common polyamine metabolism in their early steps. The hy- few genera of the plant family Solanaceae, including Hyoscyamus, droxylase gene from Hyoscyamus niger has been introduced into Duboisia, Atropa, and Scopolia, are able to produce biologically Atropa belladonna, a typical hyoscyamine-rich tropane alkaloid- active nicotine and rooot alkaloids simultaneously 1—3.

Cryopreservation method for hairy roots of Hyoscyamus muticus was developed to replace laborious subculturing, and although the post-thaw gyoscymous rates remained low, the expression of transgene remained unaltered in cryopreserved roots. As stated by many researchers, the lack of reproducibility is a factor that limits routine application of cryopreservation Reed et al.

Introduction Plant Cell Cultures Plants offer an enormous potential for humans in applications such as novel drugs, biopolymers, high-value chemical compounds, food and feed. HPLC as described Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes and Agrobacterium tumefaciens containing rol A, B, C hyosfymous only.

Slow freezing method described by Schmale et al.